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Nikon LIPSI for High-Content imaging
Nikon LIPSI for High-Content imaging
Spinning-disk based confocal, or epi-fluorescence widefield, High-content / High-Throughput Microscope with Robot-loader for multiwell plates.
Key Features:
Stage, robot and plates storage inside a closed incubator (37ºC, CO2)
up to 4-5 channels imaging in confocal or widefield epifluorescence
fast imaging with parallel cameras
Multi user / multiplexed parallel experiments with a task scheduler
Contact us about this instrument: microscopy(at)irbbarcelona(dot)org
Overview (outside)
Overview (outside)
Plate transfer from Hotels to Stage inside incubator
Overview (inside)
Overview (inside)
Insights into Robot loading inside incubator
High-Throughput Imaging for Drug Screening
Left: 384 plate layout, 294 wells imaged in 22', 2 channels 10x magnification. Right-Top: full-well segmentation, Right-Bottom: Analysis visualization heatmap.
(Images/Project: Luis Nieto, Bárbara Barbosa, Mayor-Ruiz group, IRB Barcelona. Anna Lladó, ADMCF)
3D cultures, in HCS
3D cultures, in HCS
High-content imaging in multiwell plates
3D organoids
3D organoids
3D fluorescence, live imaging. Samples: Joffrey Pelletier (IRB Barcelona)
3D Analysis
3D Analysis
3D multicomponent segmentation/analysis. Analysis: S. Tosi (ADMCF)
Technical details & Specifications
Multiscale: from 4x dry to high resolution 60x water, water dispenser for high-resolution automation
Plate formats: most multiwell plates formats can be included, plastic or glass bottom, transparent or dark.
Smart imaging: Multiscale imaging with primary scan (pre-find targets) and secondary high resolution scan
Long term imaging: Imaging up to 1 week at fast pace (continuous throughout plate) or 2 weeks at slow pace (e.g. 1 or 2 reads per day).
Fluorescence: widefield with LEDs or spinning disk/laser widefield with 6 lasers (405, 445, 488, 515, 561, 640).
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