Advanced Optical & Fluorescence Microscopy & BioImage Analysis Course, Oct 24-26, 2023
ADM offers a 7.5 hours course on Advanced Optical and Fluorescence Microscopy and BioImage Analysis.
This course targets research staff who need Bioimaging: Phd students, Postdocs and research staff from IRB Barcelona and institutions at the PCB. The course is free and will be held in person.
Registration is based on first come first served.
Attendance is strongly recommended for 1st year Phd candidates at IRB, who have guaranteed seat in the course.
The course capacity is about 30 seats. Attendance is expected for all days of the course for people who register.
Registration will be prioritized this way:
- IRB research staff: automatic confirmation during the week 2-6 OCT, first-come first-served.
- research staff from public institutions at the PCB: from OCT 17th, upon availability of seats, first-come first-served and priority to junior research staff who has not attended earlier editions.
- research staff from other public institutions and private entities: upon availability.
Access the Registration Form HERE
Speakers/Trainers:
Lídia Bardia, Anna Lladó, Nikolaos Giakoumakis, Chong Zhang, Julien Colombelli
Where: Sala Dolors Aleu, Parc Científic de Barcelona PCB (C. Baldiri Reixac, 6. 08028 Barcelona)
PRELIMINARY Program (Subject to changes)
OCT 24th, 15h-16h30 Trainer: Julien Colombelli
Day 1 / SESSION 1 Image Formation and 3D Imaging
Optical Microscopy, Optical Contrasts
Fluorescence: Basics, photophysics of fluorescence emission
Laser Scanned Confocal Microscopy principles
Detectors
Resolution in Optical Microscopy
3D Imaging contrasts: Speed or Resolution ?
Multiphoton Microscopy, Spinning Disk, Airyscan
More Optical Sectioning modalities: special techniques
Second Harmonic Generation, Spectral Detection, TIRF
Hyperspectral imaging
Laser Safety: basic concepts
OCT 24th, 17h-18h30 Trainers: Nikos Giakoumakis, Julien Colombelli
Day 1 / SESSION 2 Nano-scale & Mesoscopy/Lightsheet
Super resolution (SR) / Nanoscopy
Resolution concepts, revisited with SR
SIM: Structured Illumination Microscopy
ISM: Image Scanning Microscopy, including Airyscan
Expansion Microscopy
SMLM: Single Molecule Localization Microscopy
PALM, STORM, Paint etc...
SMLM Analysis
STED nanoscopy
Meso-scale Imaging, Mesoscopy, Lightsheet Fluorescence Microscopy
LSFM/SPIM: Single Plane Illumination Microscopy
Lightsheet imaging principles
Live Imaging with LSFM and variants: SPIM/DSLM, Oblique Plane Microscopy (OPM)
Lightsheet imaging with Optical Clearing for large samples
Large Samples Imaging with cleared organs
OCT 25th, 9h30-11h Trainers: Anna Lladó, Nikos Giakoumakis
Day 2 / SESSION 3 Molecular Imaging and High-Content
Correlative Light to Electron Microscopy (CLEM)
Photomanipulation: Fluorescence Recovery After Photobleaching (FRAP)
How to tackle molecular kinetics with FRAP ?
Data Analysis, EasyFRAP
FRET / FLIM
Protein Interaction with FRET
Acceptor Photobleaching, Sensitized Emission and Ratio Imaging
Lifetime of a fluorophore
Lifetime-based FRET
sm: single molecule techniques:
Time Correlated Single Photon Counting (TCSPC)
sm dynamics for FRET.
High-Content and High-Throughput Microscopy
Multidimensional Acquisition
Preparation of a High-content experiment
High-content Confocal Imaging
Intelligent Imaging
OCT 25th, 11h30-13h Trainer: Chong Zhang
Day 2 / SESSION 4 BioImage Analysis in Life science - Part I
Introduction to BioImage Analysis (BIA): motivations and aims
Digital Images & (image) Data Quality
If time allows: Practical Introduction to ImageJ /Fiji
OCT 26th, 15h-16h30 Trainer: Chong Zhang
Day 5 BioImage Analysis in Life science - Advanced, Part II
Image processing fundamentals: filters, image segmentation, binary mask processing, ...
Machine Learning
Deep Learning