Advanced Optical & Fluorescence Microscopy & BioImage Analysis Course, Oct 23, 27 and 30, 2025
ADM offers an approx. 9hours course on Advanced Optical and Fluorescence Microscopy and BioImage Analysis.
Where: Sala Serratosa, PCB, October 23, 27 and 30 (9h30-13h00 each day, with break)
This course targets research staff who need Bioimaging: Phd students, Postdocs and research staff from IRB Barcelona and institutions at the PCB. The course is free and will be held in person.
Registration is based on first come first served.
Attendance is strongly recommended for 1st year Phd candidates at IRB, who have guaranteed seat in the course.
The course capacity is about 50 seats. Attendance is expected for both days of the course for people who register.
Registration will be prioritized this way:
- IRB research staff: automatic confirmation, first-come first-served.
- research staff from public institutions at the PCB: upon availability of seats, first-come first-served and priority to junior research staff who has not attended earlier editions.
- research staff from other public institutions and private entities: upon availability, confirmation by November 25th.
(Access the registration Form HERE)
Speakers/Trainers: Lídia Bardia, Anna Lladó, Nikolaos Giakoumakis, Chong Zhang, Julien Colombelli
PRELIMINARY Program (Subject to changes)
Thu 23rd October
9h30-11h00 Trainer: Julien Colombelli
Day 1 / SESSION 1 Image Formation and 3D Imaging
Optical Microscopy, Optical Contrasts
Fluorescence: Basics, photophysics of fluorescence emission
Laser Scanned Confocal Microscopy principles
Resolution in Optical Microscopy
3D Imaging contrasts: Speed or Resolution ?
More Optical Sectioning modalities: special techniques
break 15 min
11h20-13h00 Trainers: Nikolaos Giakoumakis
Day 1 / SESSION 2 Nano-scale Imaging
Super resolution (SR) / Nanoscopy
Resolution concepts, revisited with SR
SIM: Structured Illumination Microscopy
ISM: Image Scanning Microscopy, including Airyscan
Expansion Microscopy
SMLM: Single Molecule Localization Microscopy
PALM, STORM, Paint etc...
SMLM Analysis
STED nanoscopy
Correlative Light to Electron Microscopy (CLEM)
Mon 27th October
9h30-11h00 Trainer: Julien Colombelli, Lídia Bardia
SESSION 3
Meso-scale Imaging (Mesoscopy), Lightsheet Fluorescence Microscopy
LSFM/SPIM: Single Plane Illumination Microscopy & Lightsheet imaging principles
Live Imaging with LSFM and variants: SPIM/DSLM, Oblique Plane Microscopy (OPM)
Optical Clearing and Lightsheet imaging for large cleared samples
break 15 min
11h20-13h00 Trainers: Anna Lladó, Nikolaos Giakoumakis
SESSION 4 Molecular Imaging and High-Content/High-Throughput
Photomanipulation: Fluorescence Recovery After Photobleaching (FRAP)
How to tackle molecular kinetics with FRAP ?
Data Analysis, EasyFRAP
FRET / FLIM
Protein Interaction with FRET, Acceptor Photobleaching, Sensitized Emission and Ratio Imaging
Lifetime of a fluorophore, Lifetime-based FRET
sm: single molecule techniques:
Time Correlated Single Photon Counting (TCSPC), sm dynamics for FRET.
break 15 min
High-Content and High-Throughput Microscopy
Multidimensional Acquisition
Preparation of a High-content experiment
High-content Confocal Imaging
Intelligent Imaging
Thu 30th October
9h30-13h00 Trainer: Chong Zhang
SESSION 5 BioImage Analysis in Life science
Introduction to BioImage Analysis (BIA): motivations and aims
Digital Images & (image) Data Quality
Introduction to Open-source BIA Software, ImageJ /Fiji etc..
11h00 break
Image processing fundamentals: filters, image segmentation, binary mask processing, ...
Machine Learning
Deep Learning