Advanced Optical & Fluorescence Microscopy & BioImage Analysis Course, Dec 2 & 5, 2024
ADM offers a 7 hours course on Advanced Optical and Fluorescence Microscopy and BioImage Analysis.
Where: Sala Serratosa, PCB, December 2 (9h30-14h00) and 5th (9h30-14h00)
This course targets research staff who need Bioimaging: Phd students, Postdocs and research staff from IRB Barcelona and institutions at the PCB. The course is free and will be held in person.
Registration is based on first come first served.
Attendance is strongly recommended for 1st year Phd candidates at IRB, who have guaranteed seat in the course.
The course capacity is about 50 seats. Attendance is expected for both days of the course for people who register.
Registration will be prioritized this way:
- IRB research staff: automatic confirmation, first-come first-served.
- research staff from public institutions at the PCB: upon availability of seats, first-come first-served and priority to junior research staff who has not attended earlier editions.
- research staff from other public institutions and private entities: upon availability, confirmation by November 25th.
THIS COURSE IS FULLY BOOKED ! (Access the registration Form HERE)
Speakers/Trainers: Lídia Bardia, Anna Lladó, Nikolaos Giakoumakis, Chong Zhang, Julien Colombelli
PRELIMINARY Program (Subject to changes)
DEC 2nd, 9h30-11h00 Trainer: Julien Colombelli
Day 1 / SESSION 1 Image Formation and 3D Imaging
Optical Microscopy, Optical Contrasts
Fluorescence: Basics, photophysics of fluorescence emission
Laser Scanned Confocal Microscopy principles
Detectors
Resolution in Optical Microscopy
3D Imaging contrasts: Speed or Resolution ?
Multiphoton Microscopy, Spinning Disk, Airyscan
More Optical Sectioning modalities: special techniques
Second Harmonic Generation, Spectral Detection, TIRF
Hyperspectral imaging
break 30min
DEC 2nd, 11h30-14h00 Trainers: Nikolaos Giakoumakis, Lídia Bardía, Julien Colombelli
Day 1 / SESSION 2 Nano-scale & Mesoscopy/Lightsheet
Super resolution (SR) / Nanoscopy
Resolution concepts, revisited with SR
SIM: Structured Illumination Microscopy
ISM: Image Scanning Microscopy, including Airyscan
Expansion Microscopy
SMLM: Single Molecule Localization Microscopy
PALM, STORM, Paint etc...
SMLM Analysis
STED nanoscopy
Correlative Light to Electron Microscopy (CLEM)
12h45 short break
SESSION 3
Meso-scale Imaging (Mesoscopy), Lightsheet Fluorescence Microscopy
LSFM/SPIM: Single Plane Illumination Microscopy
Lightsheet imaging principles
Live Imaging with LSFM and variants: SPIM/DSLM, Oblique Plane Microscopy (OPM)
Lightsheet imaging with Optical Clearing for large samples
Large Samples Imaging with cleared organs
DEC 5th, 9h30-11h Trainers: Anna Lladó, Nikolaos Giakoumakis
Day 2 / SESSION 4 Molecular Imaging and High-Content/High-Throughput
Photomanipulation: Fluorescence Recovery After Photobleaching (FRAP)
How to tackle molecular kinetics with FRAP ?
Data Analysis, EasyFRAP
FRET / FLIM
Protein Interaction with FRET
Acceptor Photobleaching, Sensitized Emission and Ratio Imaging
Lifetime of a fluorophore
Lifetime-based FRET
sm: single molecule techniques:
Time Correlated Single Photon Counting (TCSPC)
sm dynamics for FRET.
High-Content and High-Throughput Microscopy
Multidimensional Acquisition
Preparation of a High-content experiment
High-content Confocal Imaging
Intelligent Imaging
break 30min
DEC 5th, 11h30-14h00 Trainer: Chong Zhang
Day 2 / SESSION 5 BioImage Analysis in Life science
Introduction to BioImage Analysis (BIA): motivations and aims
Digital Images & (image) Data Quality
Introduction to Open-source BIA Software, ImageJ /Fiji etc..
12h45 short break
Image processing fundamentals: filters, image segmentation, binary mask processing, ...
Machine Learning
Deep Learning