Fluorescence Recovery After PhotoBleaching (FRAP) is available on 4 of our instruments:

- SP2 Spectral Confocal

- SP5 Spectral Confocal

- Andor Revolution FRAPPA

- WideField FRAP on Axiovert 200M (custom platform)

To get more details about FRAP, please contact us microscopy (at) irbbarcelona.org

FRAP Technique and Analysis

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We perform data preparation and processing with ImageJ for measurements of FRAP intensities.

We preferentially use the Igor plugin developed by Kota Miura (CMCI, EMBL) to perform FRAP curves fitting and analysis.