Eclipse Nikon Widefield Microscopes

Upright and inverted research-level microscopes from Nikon Eclipse series of instruments for Imaging of fixed

and live samples, from slides to culture dishes.

The following Illumination contrasts are available:

- WideField Epifluorescence: Generic Fluorescence Imaging in reflection mode without Optical Sectioning

See here for an excellent general introduction about Fluorescence Microscopy

- BrightField transmission: Generic high resolution imaging of absorbing specimens, including

see here for details about Köhler Illumination

- Phase Contrast: To improve visibility and contrast in transparent specimens, see here for details & examples.

- Differential Interference Contrast (DIC): For high-resolution enhancement of transparent specimens, see here for details & examples.

- Transmitted DarkField: To enhance contrast with refracting specimens, see here for details & examples.

- Polarized Light Microscopy: Contrast enhancement for birefringent specimens, see here for details & examples.

Contact Lídia Bardia and Anna Lladó to use these instrument: microscopy(at)irbbarcelona.org

Nikon E1000

The Nikon E1000 is a fixed-stage upright microscope configured to image in

Brightfield transmitted light, phase contrast, Dark field and epifluorescence modes

Technical Features

The Nikon E800 is a fixed-stage upright microscope configured to image in

transmitted light and epifluorescence modes

Technical Features

The Nikon E600 is a fixed-stage upright microscope configured to image in

transmitted light, phase contrast, DIC, Polarized-light and epifluorescence modes

Technical Features

Polarized Light microscopy: contrast-enhanced images produced by

the birefringence of specimens and

obtained by the recombination and interference of two light paths with split polarizations.

Example here: Sugar crystals.

The Nikon TE200 is a fixed-stage inverted microscope configured to image in

transmitted light, phase contrast and epifluorescence modes

Technical Features