Eclipse Nikon Widefield Microscopes

Upright and inverted research-level microscopes from Nikon Eclipse series of instruments for Imaging of fixed
and live samples, from slides to culture dishes.

The following Illumination contrasts are available:

    - WideField Epifluorescence:                            Generic Fluorescence Imaging in reflection mode without Optical Sectioning        
                                                                             See
here for an excellent general introduction about Fluorescence Microscopy
 
    - BrightField transmission:                               Generic high resolution imaging of absorbing specimens, including 
                                                                                                                                                                         
  see here for details about Köhler Illumination    
       
    - Phase Contrast:                                              To improve visibility and contrast in transparent specimens,      see here for details & examples.
    - Differential Interference Contrast (DIC):         For high-resolution enhancement of transparent specimens,       see here for details & examples.
    - Transmitted DarkField:                                   To enhance contrast with refracting specimens,                         see here for details & examples.
    - Polarized Light Microscopy:                            Contrast enhancement for birefringent specimens,                     see here for details & examples.

Contact Lídia Bardia and Anna Lladó to use these instrument:  microscopy(at)irbbarcelona.org




    Nikon E1000


The Nikon E1000 is a fixed-stage upright microscope configured to image in
Brightfield transmitted light, phase contrast, Dark field and epifluorescence modes

Technical Features

 FeatureSpecifications
 Comments
Fluorescence
Filters
Cy5:   Ex.    607/70
          BS     660
          Em.   692/40
FITC   Ex.    465-485
          BS     505
          Em.   515-555
UV-2A Ex.    330-380
          BS     400
          Em.   420
G2A    Ex.    510-560
          BS     575
          Em.   590
YFP     Ex.    500/20
          BS     515
          Em.   535/30
     Cy5-like (you need to use the camera for watching it)

      GFP-like


      Dapi-like


      RFP-like


      YFP-like
 Objective lenses4x/0.13       Plan Fluor   PhL DL   16.4  WD
10x/0.3       Plan Fluor   DIC L      16     WD
20x/0.5       Plan Fluor   DIC M      2.1   WD
40x/0.75     Plan Fluor   DIC M      0.72  WD
60x/1.4oil   Plan Apo     Ph3 DM    0.21  WD
100x/1.3oil  Plan Fluor   Ph3 DLL   0.20  WD

 
 Condenser Inserts
BF(A); Ph1; Ph2; Ph3; DF
 
 CameraCoolSnap fx Photometrics
 12 bit camera
 Acquisition SoftwareMetamorph v 6.3r0
 Olympus

    Nikon E800


The Nikon E800 is a fixed-stage upright microscope configured to image in
transmitted light and epifluorescence modes

Technical Features

 FeatureSpecifications
 Comments
Fluorescence
Filters
B2-A:    Ex.    450-490
            BS     505
            Em.   520
FITC     Ex.    465-485
            BS     505
            Em.   515-555
UV-2A    Ex.    330-380
             BS     400
             Em.   420
G2A      Ex.    510-560
            BS     575
            Em.   590
DsRed   Ex.    545/30
            BS     570
            Em.   620/60
      GFP-like (but can          cross with red)

      GFP-like


      Dapi-like


      RFP-like


      RFP-like
Objective lenses
4x/0.13     Plan Fluor    PhL DL     16.4   WD
10x/0.3     Plan Fluor    Ph1 DLL    16     WD
20x/0.5     Plan Fluor    Ph1 DLL    2.1    WD
40x/0.75    Plan Fluor   Ph2 DLL    0.72  WD
60x/1.4oil  Plan Apo      DIC H      0.21  WD
100x/1.3oil Plan Fluor   DIC H       0.20 WD
 
 Condenser InsertsBF
 
 CameraOlympus DP72
 8 bit camera/ color camera
 Acquisition Software
Cell F
 Olympus


    Nikon E600


The Nikon E600 is a fixed-stage upright microscope configured to image in
transmitted light, phase contrast, DIC, Polarized-light and epifluorescence modes

Technical Features

 FeatureSpecifications
 Comments
Fluorescence
Filters
CFP:     Ex.    436/20
            BS     455
            Em.   480/40
FITC     Ex.    465-485
            BS     505
            Em.   515-555
UV-2A   Ex.    330-380
            BS     400
            Em.   420
G2A      Ex.    510-560
            BS     575
            Em.   590

      CFP-like  


      GFP-like


      Dapi-like


      RFP-like
 Objective lenses 2x/0.06    Plan UW                    7.5    WD 
 4x/0.13    Plan Fluor                 17.1   WD
 10x/0.3    Plan Fluor    Ph1 DLL   16    WD
 20x/0.5    Plan Fluor    Ph1 DLL   2.1   WD
 40x/0.75   Plan Fluor    Ph2 DLL  0.72  WD

 100x/1.4oil Plan Apo    Ph3 DM   0.13  WD
 
 Condenser InsertsBF(A);Ph1; Ph2; Ph3; DIC H; DIC M; DIC L
 Polarized Light available
 CameraOlympus DP 72
 8 bit camera/ color camera
 Acquisition SoftwareCell F
 Olympus


    Nikon TE200


The Nikon TE200 is a fixed-stage inverted microscope configured to image in
transmitted light, phase contrast and epifluorescence modes

Technical Features

 FeatureSpecifications
 Comments
Fluorescence
Filters
B2-A:    Ex.    450-490
            BS     505
            Em.   520
FITC     Ex.    465-485
            BS     505
            Em.   515-555
UV-2A    Ex.    330-380
             BS     400
             Em.   420
G2A       Ex.    510-560
             BS     575
             Em.   590
     GFP-like (but can                 cross with red)

      GFP-like


      Dapi-like


      RFP-like


     
 Objective lenses
4x/0.13        Plan Fluor    PhL DL                 17.4   WD
10x/0.3        Plan Fluor    Ph1 DLL               16    WD
20x/0.45      Plan Fluor    ELWD-Ph1/DM      7.4    WD
40x/0.60      Plan Fluor   
ELWD-Ph2/DM      3.7-2.7   WD

 
 Condenser InsertsBF(A); PhL; Ph1; Ph2
 
 CameraOlympus DP 72
 8 bit camera/ color camera
 Software acquisitionCell D
 Olympus

















































Polarized Light microscopy: contrast-enhanced images produced by
the birefringence of specimens and
obtained by the recombination and interference of two light paths with split polarizations.
Example here: Sugar crystals.