Visitor Lab, Wet Lab

Our Facility offers access to lab instruments and a culture room for external visitors and for critical microscopy experiments
requiring liquid sample manipulation, cell culture, temperature incubation, etc...

The lab is accessible to everyone using the microscopes under general conditions, if you are external please contact us to know
more details:     microscopy(at)irbbarcelona.org

General rules:

Cell Culture:

- Under authorization, you can temporarily place your cells in our visitor's incubator.
- Cells should be tested for mycoplasma before entering the facility.

 Instrument Details
 RayPa water bath   with temperature and time control
 Visitor's incubator Innova CO-48 set at 37ºC and 5% CO2
 Lab incubator HeraCell 150 copper incubator set at 37ºC and 5% CO2
 Telstar BioStarPlus Hood Class II hood
 Liebherr Fridge and freezer Set at 5ºC and -20ºC respectively
 TS100 Eclipse Nikon microscope Equipped with: 4x, 10x and 20x phase contrast objectives and DigitalSight Nikon camera with small monitor


Small instruments:

Are freely available to anyone, find below a list with technical specifications:

 Instrument Details
 Eppendorf 5.702 Centrifuge      
 2 rotors for up to 4.400rpm available: for 24 eppendorfs or 4 to 8 falcon tubes 
 Fisher Scientific Bath Sonicator model F15049 Temperature adjustable up to 80º, timer from 1min  to infinite
 Fisher Scientific Isotemp Heating & steering plate  Temperature adjustable up to 540º, steering up to 1.200rpm
 Fisher Scientific vortex TopMix FB51024
 Continuos or pulsed vortex speed up to 40Hz
 Eppendorf Thermomixer Compact To heat and agitate up to 24 eppendorfs
 DHAUS Precision Balance To weight up to 600g
 BioRad PowerPac Basic  300V-400mA-75W power supply for running DNA gels
 SciePlas Electrophoresis cubette for DNA gels with gel casts, combs and electrodes
 Oscilloscope on demand, 2 Channels

Consumables:

- Lens cleaning tissues, ethanol, distilled water, lens immersion oils are freely available in the facility.

- Glass sample mounting material (slides, dishes) are NOT provided by the facility, please purchase your own stocks.