SP2 Spectral Confocal

Leica TCS SP2 AOBS system:
Inverted spectral confocal microscope for routine and Time-Lapse 3D/4D imaging

Contact Lídia Bardia and Anna Lladó to use this instrument:  microscopy(at)irbbarcelona.org
Technical Features

 Feature         Specifications Comments
 Laser Illumination
405nm diode laser
Argon laser (458nm-476nm-488nm-496nm-514nm)
561nm DPSS laser
594nm HeNe laser
633nm HeNe laser		 Dapi-like
 CFP; GFP; Cy2; A488-like; YFP
 RFP; Cy3; A568-like
 A594; TxRed-like
 Cy5; A647-like
 Microscope
 Leica DM IRE2
 Inverted microscope
 Acquisiton Software
 LCS 
 Detectors 3 PMTs + 1transmission PMT  
 Scanning 2D (x-y; x-t; x-z)
 3D (x-y-z; x-y-t; x-y-lambda)
 4D (x-y-z-t; x-y-t-lambda) 		 
 Resolution from 128x128 up to 4096x4096 pixels
 
 Image depth
 8-12 bit
 
 Zoom 1x-32x 
Speed 2.800Hz (bi-directional mode)
 
 Sample Mounting
  
 Stage Motorized rotary stage
 Multiposition and Mosaic options
 Widefield Fluorescence
 Filters

I3:        Ex.    BP 450-490
            BS     510
            Em.   LP 515 
N2.1:            Ex.    BP 515-560
                     BS     580
                    Em.   LP 590  

UV:        Ex.    BP 340-380
             BS     400
             Em.   LP 425 		 

 GFP-like



 RFP-like



 DAPI-like
 Objective lenses
10x/0.40 CS                      HC PL APO
         
20x/0.70 CS                      HC PL APO
   
40x/1.25-0.75 Oil CS         HCX PL APO

63x/1.40 Oil CS  PH3         HCX PL APO

63x/140-0.6 Oil Lbd BL      HCX PL APO

 
 Environment T, CO2
 

Applications

 Confocal Fixed sample scanning
 Live Imaging
 Transmission detection
 Bright Field; DIC; PH3
 FRAP/FLIP 
 FRET Sensitized emission
 Acceptor Photobleaching
 Photoactivation like PA-GFP protein
 Photoconversion From green to red like                 Dendra2 or EOS proteins