Andor 'Revolution' Spinning Disk

Inverted spinning disk microscope for fast acquisition and FRAPPA processes

Contact Lídia Bardia to use this instrument:  microscopy(at)irbbarcelona.org

Technical Features

 FeaturesSpecifications
 Comments
 Laser illumination
405nm diode laser


488nm diode laser

561nm diode laser
 For FRAP, Photoactivation and Photoconversion techniques

GFP-like

RFP-like
 Microscope Olympus IX 81
 
 Acquisition software
 iQ1 (FastLZ)

 iQ2 (FRAPPA module)
version 2.4.1

version 2.4.1

 Camera iXon EMCCD Andor DU-897
Ultra-Sensitive EMCCD camera
16um pixel size
 Resolution 512x512 pixels
 
 Image depth
 14 bit
 
 Sample mounting
  
 Stage PRIOR Motorized stage for
 X-Y multiple fields Images
 Multiposition and Mosaic
 Widefield Fluorescence
UV
GFP
TxRed
Dapi - like
GFP - like
RFP - like
 Objective lenses
10x/0.30                    UPlanFL N
         
20x/0.50                    UPlanFL N
   
40x/0.60                    LUC
PlanFL N

60x/1.42 Oil   
           Plan Apo N

100x/1.40 Oil             UPlanSApo
 





DIC60

DIC100
 Spinning disk
Yokogawa CSU-X1 (confocal spinning disk unit)
1500-10000 rpm disk, giving 300 to more than 2000 fps frame rate
 Focus control
Piezo Objective stage 0-100um Physik Instrumente
 FRAPPA Fluorescence Recovery After Photo bleach (FRAP) and Photo Activation (PA)To bleach or activate regions of interest
with user-defined times, laser lines and powers
 Fluorescence lamp
 X-Cite series 120Q
Offers rich spectral fluorescence excitation with a uniformly illuminated field of view and has 2000 hour lamp lifetime, pre-aligment and adjustable iris
Environmental conditions
 Temperature and CO2 control
 and feedback inside the whole
 incubation chamber
 from EMBL (Heidelberg -Germany)

Applications


 Fast live imaging
 
 Multiple field scanning
 
 FRAP 
 Photoactivation 
 Photoconversion